Author: Yosuke Hirotsu; Hitoshi Mochizuki; Masao Omata
Title: Double-Quencher Probes Improved the Detection Sensitivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by One-Step RT-PCR Document date: 2020_3_20
ID: 2gokv7id_23
Snippet: We concluded that the double-quencher probe (ZEN/IBFQ) reduced the background signal and lowered the Ct value compared to the single-quencher probe (TAMRA). To this end, we analyzed the fluorescent signals during the cycles of RT-PCR at three independent experiments. As expected, the doublequencher probes decreased the background FAM fluorescent signals at the baseline ( Figure 3 ). The baseline fluorescent signal of the N1 site of NIID ranged fr.....
Document: We concluded that the double-quencher probe (ZEN/IBFQ) reduced the background signal and lowered the Ct value compared to the single-quencher probe (TAMRA). To this end, we analyzed the fluorescent signals during the cycles of RT-PCR at three independent experiments. As expected, the doublequencher probes decreased the background FAM fluorescent signals at the baseline ( Figure 3 ). The baseline fluorescent signal of the N1 site of NIID ranged from 150×10 4 to 200×10 4 . However that of YCH was around or less than 50×10 4 . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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