Author: Senanayake, Savithra D.; Brian, David A.
Title: Precise large deletions by the PCR-based overlap extension method Cord-id: 147erliy Document date: 1995_1_1
ID: 147erliy
Snippet: The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
Document: The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
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