Selected article for: "amino acid and high animal"
Author: Ze Chen; Xiaofeng Xu; Xiaojun Yang; Weihao Dou; Xiufeng Jin; Haishuo Ji; Guangyuan Liu; Jianxun Luo; Hong Yin; Gao Shan
Title: DNA segment of African Swine Fever Virus first detected in hard ticks from sheep and bovine
  • Document date: 2018_12_3
    • ID: 36rq4rmg_8
    Snippet: Further analysis of two 235-bp sequences from D. silvarum and sheep showed an identity of 100% 113 between them (Figure 1B) . Using the ASFV reference genome (GenBank: AY261365.1), three single 114 nucleotide mutations were detected at the positions of 38, 76 and 108 (C38T, C76T and A108C) on these 115 two 235-bp sequences. As the 235-bp ASFV segment encodes 78 amino acids, C38T results in a single 116 amino acid mutation at the position 66 (G66D.....
    Document: Further analysis of two 235-bp sequences from D. silvarum and sheep showed an identity of 100% 113 between them (Figure 1B) . Using the ASFV reference genome (GenBank: AY261365.1), three single 114 nucleotide mutations were detected at the positions of 38, 76 and 108 (C38T, C76T and A108C) on these 115 two 235-bp sequences. As the 235-bp ASFV segment encodes 78 amino acids, C38T results in a single 116 amino acid mutation at the position 66 (G66D). C38T suggested the existence of a new ASFV strain, which 117 was different from all reported ASFV strains in NCBI GenBank database (version 197). As the D. silvarum 118 ticks and the sheep blood were collected from two different places, this new strain could infected sheep and 119 be transmitted by D. silvarum ticks. Using blast tools, the results showed that the new 235-bp segment in the 120 new ASFV strain did not contain high similar regions with sequences from other viruses, bacteria or animal 121 genomes. As this segment was highly specific to represent ASFV, our study still proved the existence of 122 ASFV in D. nuttalli, D. silvarum and sheep. 123 124

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