Selected article for: "cell medium maintain and flow cytometry"
Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies
  • Document date: 2019_3_23
    • ID: 5bw7umap_69
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint AICE to 1 cell), or CD3/CD28 Dynabeads at a 1:2.5 cell:bead ratio (Life Technologies #11131D) for 4 days. CD8+ T Cells were imaged through the spinning disk confocal microscope (Nikon Yokogawa CSU-22) one day after AICE addition. CD4+ and CD8+ T Cells numbers were quantified using a cell counter (Counte.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint AICE to 1 cell), or CD3/CD28 Dynabeads at a 1:2.5 cell:bead ratio (Life Technologies #11131D) for 4 days. CD8+ T Cells were imaged through the spinning disk confocal microscope (Nikon Yokogawa CSU-22) one day after AICE addition. CD4+ and CD8+ T Cells numbers were quantified using a cell counter (Countess II FL AMQAF1000, ThermoFisher) every other day from day 6 to day 14 after AICE activation. Fresh IL-2 containing medium was supplemented to maintain the cell concentration between 0.5-1.5 million per mL. On day 14, T cell phenotype was studied by flow cytometry using the following antibodies: anti-CCR7 (BD #561271), anti-CD45RA (BD #562885), anti-LAG-3 (BD #565720), anti-TIM-3 (BD #565558), anti-PD-1 (Biolegend #329936).

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