Selected article for: "confocal microscope and fluorescence microscope"
Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies
  • Document date: 2019_3_23
    • ID: 5bw7umap_55
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint (LifeTein) and 50 nmol 5'Cy3-labeled DNA-21mer (Bioresearch Technologies) dissolved in 50 μL PBS (phosphate buffered saline, pH7.0) was mixed into 0.5 mL ethyl acetate with dissolved PLGA (Sigma #719900), and pro.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint (LifeTein) and 50 nmol 5'Cy3-labeled DNA-21mer (Bioresearch Technologies) dissolved in 50 μL PBS (phosphate buffered saline, pH7.0) was mixed into 0.5 mL ethyl acetate with dissolved PLGA (Sigma #719900), and probe-sonicated at 7-8 W for 5 x 5 s with 10 s intervals on ice. Immediately after this, amphiphilic polymer-DNA and aqueous buffer was added following the same protocol described above. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint minutes, followed by two washes (centrifugation at 400 g x 5 minutes and resuspension in PBS) for flow cytometry with a BD LSR II. Anti-PD-L1-DNA conjugate linked through the "TCEP" strategy was loaded on DNA-scaffolded PLGA microparticles. PD-L1 overexpressed and wildtype K562 cells at 1 million/mL were respectively added with particles at 0.3 OD550 and incubated for 30 minutes at 37 o C, followed by cell nuclei staining with Hoechst 33342 (ThermoFisher #62249) and the imaging using the spinning disk confocal fluorescence microscope (Nikon Yokogawa CSU-22).

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