Author: Tesfahun Dessale; Krishna Chaithanya Batchu; Diogo Barardo; Ng Li Fang; Vanessa Yuk Man Lam; Linfan Xiao; Markus R. Wenk; Nicholas S. Tolwinski; Jan Gruber
Title: Doubling healthy lifespan using drug synergy Document date: 2017_6_21
ID: 9e3z9xdd_33
Snippet: were counted every day until all worms were dead. For RAP+RIF+ALLAN the combined 101 lifespan data was imported to Survcurv for mortality analysis and maximum lifespan 102 comparison 3,4 . We used the Gompertz survival model to determine the initial mortality rate 103 and mortality rate doubling time using the following equation Freshly prepared bacteria (OP50 E. coli) were spotted on 94 cm NGM agar plates on the 115 previous evening and allowed .....
Document: were counted every day until all worms were dead. For RAP+RIF+ALLAN the combined 101 lifespan data was imported to Survcurv for mortality analysis and maximum lifespan 102 comparison 3,4 . We used the Gompertz survival model to determine the initial mortality rate 103 and mortality rate doubling time using the following equation Freshly prepared bacteria (OP50 E. coli) were spotted on 94 cm NGM agar plates on the 115 previous evening and allowed to dry. Synchronized, young adult worms were transferred to 116 fresh plates. For maintaining synchronized populations, FUdR was added to NGM media. 117
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