Author: Tesfahun Dessale; Krishna Chaithanya Batchu; Diogo Barardo; Ng Li Fang; Vanessa Yuk Man Lam; Linfan Xiao; Markus R. Wenk; Nicholas S. Tolwinski; Jan Gruber
Title: Doubling healthy lifespan using drug synergy Document date: 2017_6_21
ID: 9e3z9xdd_37
Snippet: by Agilent Bioanalyzer 2100. For library preparation an amount of 2µg of total RNA per 124 sample was processed using Illumina's RNA Sample Prep Kit following the manufacturer's 125 instruction (Illumina, San Diego, CA, USA). All single drugs were processed together and 126 multiplexed onto one lane to minimize batch effect. The different drug combinations were 127 processed into two multiplexed lanes. In total, we ran three lanes in parallel wi.....
Document: by Agilent Bioanalyzer 2100. For library preparation an amount of 2µg of total RNA per 124 sample was processed using Illumina's RNA Sample Prep Kit following the manufacturer's 125 instruction (Illumina, San Diego, CA, USA). All single drugs were processed together and 126 multiplexed onto one lane to minimize batch effect. The different drug combinations were 127 processed into two multiplexed lanes. In total, we ran three lanes in parallel with 20 samples 128 in each and we had untreated N2 controls in each lane. Libraries were sequenced using 129
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