Author: Yosuke Hirotsu; Hitoshi Mochizuki; Masao Omata
Title: Double-Quencher Probes Improved the Detection Sensitivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by One-Step RT-PCR Document date: 2020_3_20
ID: 2gokv7id_19
Snippet: We set the threshold value to 0.2 in all assays to determine the threshold cycle (Ct). Fluorescence amplification signals were detected using 10,000 copies, 1000 copies, and 100 copies of DNA positive control by all CDC, NIID, and YCH assays ( Figure 2 ). With the use of 10 copies of DNA positive control, amplification signals by CDC-N2, YCH-N1, YCH-N2, and NIID-N2 were detected ( Figure 2 ). All of these assays could not detect using 1 copy of D.....
Document: We set the threshold value to 0.2 in all assays to determine the threshold cycle (Ct). Fluorescence amplification signals were detected using 10,000 copies, 1000 copies, and 100 copies of DNA positive control by all CDC, NIID, and YCH assays ( Figure 2 ). With the use of 10 copies of DNA positive control, amplification signals by CDC-N2, YCH-N1, YCH-N2, and NIID-N2 were detected ( Figure 2 ). All of these assays could not detect using 1 copy of DNA positive control ( Figure 2 ).
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