Dynamics of viral index infections in novel hosts

Nardus Mollentze; Daniel G. Streicker; Pablo R. Murcia; Katie Hampson; Roman Biek

Differences between experiments were accommodated by including variables for dose, the 477 inoculation site, and whether the inoculum consisted of brain material or was derived from salivary 478 glands/saliva, along with a random effect distinguishing between experiments to accommodate any 479 remaining differences. Because the doses encountered in these experiments differed over several 480 orders of magnitude and the effects of increasing dose is assumed to decrease (saturate) at very 481 large doses, this variable was included in its log-transformed form. The varying inoculation routes 482 encountered were summarised as a 'proportional inoculation distance', representing the relative 483 distance between the inoculation site and the brain (the primary site of rabies virus replication). 484 as subcutaneous inoculations to the head, and were subsequently scaled to range between 0 642 (intracranial, head) and 1 (subcutaneous, head). See supplementary document S1 for details. 643 . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.09.033928 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.09.033928 doi: bioRxiv preprint