Author: Guo, Lian Yu; Joo, Eun Ji; Son, Kun Ho; Jeon, Su Jin; Jang, Sehyun; Shin, Eun Myoung; Zhou, Hong Yu; Kim, Yeong Shik
Title: Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells Cord-id: 1lu2zrur Document date: 2009_11_8
ID: 1lu2zrur
Snippet: Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC(50) value of cimiside E on gastric cancer cells for 24 h was 14.58 µM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 µM) and G2/M phase at hig
Document: Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC(50) value of cimiside E on gastric cancer cells for 24 h was 14.58 µM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 µM) and G2/M phase at higher concentrations (60 and 90 µM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
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