Author: Noam Shental; Shlomia Levy; Shosh Skorniakov; Vered Wuvshet; Yonat Shemer-Avni; Angel Porgador; Tomer Hertz
Title: Efficient high throughput SARS-CoV-2 testing to detect asymptomatic carriers Document date: 2020_4_20
ID: bby9hls2_4
Snippet: Following genome-extraction and PCR amplification of all pools, a decoding algorithm was used to identify carriers that were then tested individually for verification. The total time for pooling 384 samples into 48 pools using a basic liquid dispensing robot (Arise EZMate-601) was <5 hours, and was performed in a standard BSL-2 laboratory, since individual samples were diluted in a lysis buffer that inactivates all viral particles......
Document: Following genome-extraction and PCR amplification of all pools, a decoding algorithm was used to identify carriers that were then tested individually for verification. The total time for pooling 384 samples into 48 pools using a basic liquid dispensing robot (Arise EZMate-601) was <5 hours, and was performed in a standard BSL-2 laboratory, since individual samples were diluted in a lysis buffer that inactivates all viral particles.
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