Author: GE, JINYING; WEN, ZHIYUN; WANG, XIJUN; HU, SEN; LIU, YONGGANG; KONG, XIANGANG; CHEN, HUALAN; BU, ZHIGAO
Title: Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cellâ€Entry Assay Cord-id: 5t4cn3ts Document date: 2006_11_17
ID: 5t4cn3ts
Snippet: abstract: We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARSâ€CoV in trans. The neutralization and infection blocking tests showed that the VSV Δ G*SG and SARSâ€CoV reacted similarly to SARSâ€CoV specific antiserum, suggesting the VSVΔ G*SG can be a safe replacement of the live SARSâ€CoV for neutralization test and cellâ€entry assay.
Document: abstract: We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARSâ€CoV in trans. The neutralization and infection blocking tests showed that the VSV Δ G*SG and SARSâ€CoV reacted similarly to SARSâ€CoV specific antiserum, suggesting the VSVΔ G*SG can be a safe replacement of the live SARSâ€CoV for neutralization test and cellâ€entry assay.
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