Selected article for: "cleavage activity and trans cleavage"

Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
  • Document date: 2020_4_14
  • ID: n5kpvsvg_7
    Snippet: Structural analysis of LbCas12a shows that it contains a single RuvC domain, which processes precursor crRNA into mature crRNA, cleaves target dsDNA or ssDNA (referred here as activators), and executes nonspecific cleavage afterwards. 19, 20 Therefore, we were interested in understanding the effects of these modified crRNAs on cis-cleavage compared to the wild-type crRNA, as well as how cis-cleavage activity correlates to the trans-cleavage activ.....
    Document: Structural analysis of LbCas12a shows that it contains a single RuvC domain, which processes precursor crRNA into mature crRNA, cleaves target dsDNA or ssDNA (referred here as activators), and executes nonspecific cleavage afterwards. 19, 20 Therefore, we were interested in understanding the effects of these modified crRNAs on cis-cleavage compared to the wild-type crRNA, as well as how cis-cleavage activity correlates to the trans-cleavage activity. Towards this, we carried out an in vitro cis-cleavage assay for various 3'-end and 5'-end modifications. We noticed that the cis-cleavage activity was either similar or marginally improved with most 3'-end modifications while the 5'-end modifications showed either similar or slightly reduced activity. This phenomenon suggests that the trans-cleavage activity is commensurate with the cis-cleavage activity .

    Search related documents:
    Co phrase search for related documents
    • cis cleavage activity and trans cleavage activity: 1, 2, 3, 4, 5, 6, 7
    • cis cleavage activity and vitro cis cleavage assay: 1
    • cis cleavage activity and wild type: 1, 2
    • cis cleavage activity and wild type crrna: 1
    • cis cleavage and ssdna dsdna target: 1
    • cis cleavage and trans cleavage: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • cis cleavage and trans cleavage activity: 1, 2, 3, 4, 5, 6, 7, 8
    • cis cleavage and vitro cis cleavage assay: 1
    • cis cleavage and wild type: 1, 2
    • cis cleavage and wild type crrna: 1
    • cis cleavage assay and trans cleavage: 1, 2
    • cis cleavage assay and trans cleavage activity: 1
    • cis cleavage assay and vitro cis cleavage assay: 1
    • cis cleavage assay and wild type: 1