Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism

Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla

NMR spectra were measured on a Varian INOVA 500 ( 1 H at 500 MHz, 13 C at 125 MHz), a Varian 400 ( 1 H at 400 MHz, 13 C at 100 MHz), or a Varian INOVA 600 MHz ( 1 H at 500 MHz, 13 C at 150 MHz) magnetic resonance spectrometer, as noted. 1 H chemical shifts were reported relative to the residual solvent peak (CDCl3= 7.26 ppm; MeOD = 3.31 ppm) as follows: chemical shift (δ) [multiplicity (s = singlet, brs=broad singlet, d = doublet, t = triplet, q = quartet, dd = doublet of doublet, m = multiplet), coupling constant(s) in Hz, integration]. 13 C chemical shifts were reported relative to the residual deuterated solvent 13 C signals (CDCl3 = 77.16 ppm, MeOD = 49.00 ppm) and rounded to one decimal places. Infrared spectra were recorded on a Nicolet iS50 FT/IR Spectrometer at the Stanford Nano Share Facilities and were reported in wavenumbers (cm -1 ). Optical rotation data were obtained using a JASCO DIP were reported as [a] 20 D (c = grams/100 mL, solvent), where D indicates the sodium D line (589 nm). High resolution mass spectra were obtained on an Agilent 6545 QTof mass spectrometer at the Metabolic Chemistry Analysis Center at Stanford University.