Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_71
Snippet: To a solution of 5 (60 mg,0.16 mmol, 1 equiv.) in anhydrous CH2Cl2 (2 mL) was added Et3N (44 µL, 0.32 mmol, 2 equiv.) at 0 °C, followed by the dropwise addition of 0.2 mL MsCl stock solution (0.26 mmol, 1.6 equiv.; Stock solution was prepared by diluting 0.1 mL MsCl with CH2Cl2 to 1 mL). After 20 min at 0 ºC, the reaction mixture was quenched with 2 mL saturated aqueous solution of NH4Cl, extracted with 3 ´ 3 mL CH2Cl2, dried over anhydrous N.....
Document: To a solution of 5 (60 mg,0.16 mmol, 1 equiv.) in anhydrous CH2Cl2 (2 mL) was added Et3N (44 µL, 0.32 mmol, 2 equiv.) at 0 °C, followed by the dropwise addition of 0.2 mL MsCl stock solution (0.26 mmol, 1.6 equiv.; Stock solution was prepared by diluting 0.1 mL MsCl with CH2Cl2 to 1 mL). After 20 min at 0 ºC, the reaction mixture was quenched with 2 mL saturated aqueous solution of NH4Cl, extracted with 3 ´ 3 mL CH2Cl2, dried over anhydrous Na2SO4, concentrated, loaded on a 4g SiO2 flash cartridge, and purified with a linear gradient 50-95% EtOAc in hexanes to afford the compound 8 (53 mg, 0.11 mmol, 72%) as pale yellow oil.
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