Author: Winchell, Jonas M; Thurman, Kathleen A; Mitchell, Stephanie L; Thacker, W Lanier; Fields, Barry S
Title: Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation. Cord-id: 5yqcdeu2 Document date: 2008_1_1
ID: 5yqcdeu2
Snippet: We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
Document: We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
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