Author: Haran Shani-Narkiss; Omri David Gilday; Nadav Yayon; Itamar Daniel Landau
Title: Efficient and Practical Sample Pooling High-Throughput PCR Diagnosis of COVID-19 Document date: 2020_4_7
ID: 6ji8dkkz_5
Snippet: Step RT-qPCR) is performed on viral RNA and human control 5 . Briefly, in RT-qPCR reaction, the extracted RNA is first reverse transcribed to a double stranded cDNA template. Next, a reaction is repeated in cycles, amplifying the target cDNA fragment exponentially, by doubling the amount of that fragment in each cycle. In a typical qPCR reaction this amplification is repeated for ~42 cycles. The cycle in which the fluorescent signal crosses a cer.....
Document: Step RT-qPCR) is performed on viral RNA and human control 5 . Briefly, in RT-qPCR reaction, the extracted RNA is first reverse transcribed to a double stranded cDNA template. Next, a reaction is repeated in cycles, amplifying the target cDNA fragment exponentially, by doubling the amount of that fragment in each cycle. In a typical qPCR reaction this amplification is repeated for ~42 cycles. The cycle in which the fluorescent signal crosses a certain threshold is linked to the starting concentration of the target cDNA.
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