Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_30
Snippet: We addressed whether viral DNA replication is needed for PKR degradation. We mock 299 infected or infected CMT93 cells with MAV-1 at an MOI of 10 and treated them with cytosine 300 arabinoside (araC) at the time of infection to inhibit DNA synthesis (69, 70) . This would allow 301 the virus to infect the cell and produce early viral proteins, but would inhibit viral DNA 302 replication and prevent late protein synthesis. We collected lysates at 2.....
Document: We addressed whether viral DNA replication is needed for PKR degradation. We mock 299 infected or infected CMT93 cells with MAV-1 at an MOI of 10 and treated them with cytosine 300 arabinoside (araC) at the time of infection to inhibit DNA synthesis (69, 70) . This would allow 301 the virus to infect the cell and produce early viral proteins, but would inhibit viral DNA 302 replication and prevent late protein synthesis. We collected lysates at 20 and 40 hpi and analyzed 303 them by immunoblot. We confirmed that araC treatment resulted in no late protein synthesis by 304 performing an immunoblot for late virion proteins (Fig. S8 ). In samples treated with araC, PKR 305 degradation was seen at 20 and 40 hpi ( We have demonstrated here that PKR is antiviral in MAV-1 infections of cultured cells. 312
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