Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR)

Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler

Instead, we showed that PKR depletion is inhibited by proteasome inhibitors, implicating 315 proteasomal degradation of PKR. Several lines of evidence suggest that the degradation is due to 316 a viral early function. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/584680 doi: bioRxiv preprint antiviral activity during infection (10, 72), MAV-1 does not produce such VA RNAs, and how 322 MAV-1 infection is affected by PKR is first described in this report. When we infected PKR -/-323 MEFs with MAV-1, viral DNA yields were 5 to 6 times higher than viral DNA yields from wild 324 type MEFs (Fig. 1) , indicating that PKR plays an antiviral role during MAV-1 infection. At 48 325 hpi, the viral DNA yield from the N-MEFs was nearly 4 times higher than the C-MEFs, but by 326 72 hpi the viral DNA yields from both types of PKR -/-MEFs were similar to each other and 327