Author: Zhang, Haijun; Rostami, Mahboubeh R.; Leopold, Philip L.; Mezey, Jason G.; O’Beirne, Sarah L.; Strulovici-Barel, Yael; Crystal, Ronald G.
Title: Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium Cord-id: 7ldddyq5 Document date: 2020_7_15
ID: 7ldddyq5
Snippet: Rationale: Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease (COVID-19), a predominantly respiratory illness. The first step in SARS-CoV-2 infection is binding of the virus to ACE2 (angiotensin-converting enzyme 2) on the airway epithelium. Objectives: The objective was to gain insight into the expression of ACE2 in the human airway epithelium. Methods: Airway epithelia sampled by fiberoptic bronchoscopy of trachea, large airway epithelia
Document: Rationale: Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease (COVID-19), a predominantly respiratory illness. The first step in SARS-CoV-2 infection is binding of the virus to ACE2 (angiotensin-converting enzyme 2) on the airway epithelium. Objectives: The objective was to gain insight into the expression of ACE2 in the human airway epithelium. Methods: Airway epithelia sampled by fiberoptic bronchoscopy of trachea, large airway epithelia (LAE), and small airway epithelia (SAE) of nonsmokers and smokers were analyzed for expression of ACE2 and other coronavirus infection–related genes using microarray, RNA sequencing, and 10x single-cell transcriptome analysis, with associated examination of ACE2-related microRNA. Measurements and Main Results: 1) ACE2 is expressed similarly in the trachea and LAE, with lower expression in the SAE; 2) in the SAE, ACE2 is expressed in basal, intermediate, club, mucus, and ciliated cells; 3) ACE2 is upregulated in the SAE by smoking, significantly in men; 4) levels of miR-1246 expression could play a role in ACE2 upregulation in the SAE of smokers; and 5) ACE2 is expressed in airway epithelium differentiated in vitro on air–liquid interface cultures from primary airway basal stem/progenitor cells; this can be replicated using LAE and SAE immortalized basal cell lines derived from healthy nonsmokers. Conclusions: ACE2, the gene encoding the receptor for SARS-CoV-2, is expressed in the human airway epithelium, with variations in expression relevant to the biology of initial steps in SARS-CoV-2 infection.
Search related documents:
Co phrase search for related documents- abundance transcript and acute respiratory syndrome: 1, 2
- abundance transcript and low abundance transcript: 1
- abundance transcript and low expression: 1
- accession number and action mechanism: 1
- accession number and acute respiratory syndrome: 1, 2, 3
- accession number and low expression: 1
- action mechanism and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- action mechanism and additional insight: 1
- action mechanism and low expression: 1
- action mechanism and low percentage: 1
- activate protease and acute respiratory syndrome: 1, 2, 3, 4
- active infection and acute respiratory syndrome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- active infection and low airway: 1
- actual percentage and acute respiratory syndrome: 1, 2
- actual percentage and low percentage: 1, 2, 3, 4
- acute respiratory syndrome and additional host protein: 1
- acute respiratory syndrome and additional insight: 1, 2, 3
- acute respiratory syndrome and low airway: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- acute respiratory syndrome and low expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date