Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_8
Snippet: We probed blots with antibodies to actin as a loading control. To our surprise, in C57BL/6 144 MEFs, PKR was almost completely depleted from lysates 24 hpi and remained depleted through 145 72 hpi ( Fig. 2A and B) . PKR was also depleted compared to mock infection at 24 and 48 hpi in 146 C57BL/6 MEFs infected at MOI 2 and 5 (Fig. S1 ). We also observed depletion of PKR in other 147 cell types. In CMT93 cells, PKR was nearly undetectable at 24 hpi.....
Document: We probed blots with antibodies to actin as a loading control. To our surprise, in C57BL/6 144 MEFs, PKR was almost completely depleted from lysates 24 hpi and remained depleted through 145 72 hpi ( Fig. 2A and B) . PKR was also depleted compared to mock infection at 24 and 48 hpi in 146 C57BL/6 MEFs infected at MOI 2 and 5 (Fig. S1 ). We also observed depletion of PKR in other 147 cell types. In CMT93 cells, PKR was nearly undetectable at 24 hpi ( Fig. 2A) . In C57BL/6 148 primary peritoneal macrophages, PKR was decreased at 48 hpi compared to mock lysates, and 149 absent in infected lysates at 72 hpi ( Fig. 2A ). This indicates that MAV-1 causes PKR depletion 150 during infection. 151
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