Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection Document date: 2020_4_14
ID: n5kpvsvg_45
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.036079 doi: bioRxiv preprint a. 10X isothermal amplification buffer: 2.5 µL b. 100 mM MgSO4: 1 µL c. 10 mM dNTP: 3.5 µL d. 10X primer mix (2 µM F3, 2 µM B3, 16 µM FIP, 16 µM BIP, LF and BF are optional): 2.5 µL e. Bst 2.0 polymerase: 1 µL f. Warmstart RT master mix: 1 µL g. RNase inhibitor, murine (NEB # M0314S): 1 µL h. Nuc.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.036079 doi: bioRxiv preprint a. 10X isothermal amplification buffer: 2.5 µL b. 100 mM MgSO4: 1 µL c. 10 mM dNTP: 3.5 µL d. 10X primer mix (2 µM F3, 2 µM B3, 16 µM FIP, 16 µM BIP, LF and BF are optional): 2.5 µL e. Bst 2.0 polymerase: 1 µL f. Warmstart RT master mix: 1 µL g. RNase inhibitor, murine (NEB # M0314S): 1 µL h. Nuclease-free water: 7.5 µL i. RNA sample: 5 µL (total volume = 25 µL)
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