Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_102
Snippet: For tests of the CPU-GSK983 combination ( Figure 5B ), A549 cells were plated overnight at 20,000 cells/well in 24-well plates in complete DMEM and incubated for 24 h at 37 °C. Cell were then treated with pyrimidine de novo synthesis and/or salvage inhibitors in DMEM supplemented with 20 µM uridine. Four hours after drug addition, cells were infected with DENV-2 virus at a MOI = 0.01. After 48 h, DENV-Luc replication was monitored by the produc.....
Document: For tests of the CPU-GSK983 combination ( Figure 5B ), A549 cells were plated overnight at 20,000 cells/well in 24-well plates in complete DMEM and incubated for 24 h at 37 °C. Cell were then treated with pyrimidine de novo synthesis and/or salvage inhibitors in DMEM supplemented with 20 µM uridine. Four hours after drug addition, cells were infected with DENV-2 virus at a MOI = 0.01. After 48 h, DENV-Luc replication was monitored by the production of Renilla luciferase, which was measured using the Renilla-Glo Luciferase Assay System (Promega) according to the specifications of the manufacturer.
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