Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_102
Snippet: For tests of the CPU-GSK983 combination ( Figure 5B ), A549 cells were plated overnight at 20,000 cells/well in 24-well plates in complete DMEM and incubated for 24 h at 37 °C. Cell were then treated with pyrimidine de novo synthesis and/or salvage inhibitors in DMEM supplemented with 20 µM uridine. Four hours after drug addition, cells were infected with DENV-2 virus at a MOI = 0.01. After 48 h, DENV-Luc replication was monitored by the produc.....
Document: For tests of the CPU-GSK983 combination ( Figure 5B ), A549 cells were plated overnight at 20,000 cells/well in 24-well plates in complete DMEM and incubated for 24 h at 37 °C. Cell were then treated with pyrimidine de novo synthesis and/or salvage inhibitors in DMEM supplemented with 20 µM uridine. Four hours after drug addition, cells were infected with DENV-2 virus at a MOI = 0.01. After 48 h, DENV-Luc replication was monitored by the production of Renilla luciferase, which was measured using the Renilla-Glo Luciferase Assay System (Promega) according to the specifications of the manufacturer.
Search related documents:
Co phrase search for related documents- complete dmem and de novo synthesis: 1, 2, 3
- complete dmem and DENV Luc replication: 1
- complete dmem and drug addition: 1, 2
- complete dmem and novo synthesis: 1, 2, 3
- complete dmem and Promega Renilla Glo Luciferase Assay System: 1, 2
- complete dmem and pyrimidine de novo synthesis: 1, 2, 3
- complete dmem and Renilla luciferase: 1, 2, 3, 4
- de novo synthesis and DENV Luc replication: 1
- de novo synthesis and drug addition: 1, 2
- de novo synthesis and novo synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- de novo synthesis and Promega Renilla Glo Luciferase Assay System: 1, 2
- de novo synthesis and pyrimidine de novo synthesis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- de novo synthesis and Renilla luciferase: 1, 2
- DENV Luc replication and drug addition: 1
- DENV Luc replication and novo synthesis: 1
- DENV Luc replication and Promega Renilla Glo Luciferase Assay System: 1
- DENV Luc replication and pyrimidine de novo synthesis: 1
- DENV Luc replication and Renilla luciferase: 1
- drug addition and Renilla luciferase: 1
Co phrase search for related documents, hyperlinks ordered by date