Selected article for: "cell viability and flow cytometry"
Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism
  • Document date: 2020_3_25
    • ID: 1zk64gsg_103
    Snippet: . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.992230 doi: bioRxiv preprint For tests of the CPU-GSK983 combination ( Figure 5B) ,For the accompanying cell viability assay, A549 cells were seeded into 24-well plates at a density of 20,000 cells/well incubated for 24 h at 37 °C. Cell were then treated .....
    Document: . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.992230 doi: bioRxiv preprint For tests of the CPU-GSK983 combination ( Figure 5B) ,For the accompanying cell viability assay, A549 cells were seeded into 24-well plates at a density of 20,000 cells/well incubated for 24 h at 37 °C. Cell were then treated with pyrimidine de novo synthesis and/or salvage inhibitors in DMEM supplemented with 20 µM uridine. Following 48h treatment, cells were harvested, and the density of viable cells was determined by flow cytometry (FSC/SSC) using a BD Accuri C6 Flow Cytometer.

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