Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_105
Snippet: Transfected A549 cells were plated at 15,000 cells/well in 96-well plates, and immediately treated with R1479, and/or, pyrimidine de novo synthesis inhibitors, and/or salvage inhibitors in complete DMEM medium supplemented with 20 µM uridine without antibiotics (Marceau et al, 2016) . Forty-eight hours after drug treatment, cells were lysed and subjected to Renilla luciferase detection, which was measured using the Renilla-Glo Luciferase Assay S.....
Document: Transfected A549 cells were plated at 15,000 cells/well in 96-well plates, and immediately treated with R1479, and/or, pyrimidine de novo synthesis inhibitors, and/or salvage inhibitors in complete DMEM medium supplemented with 20 µM uridine without antibiotics (Marceau et al, 2016) . Forty-eight hours after drug treatment, cells were lysed and subjected to Renilla luciferase detection, which was measured using the Renilla-Glo Luciferase Assay System (Promega) according to the specifications of the manufacturer.
Search related documents:
Co phrase search for related documents- cell plate and luciferase detection: 1
- complete dmem medium and dmem medium: 1, 2, 3, 4, 5, 6, 7, 8
- de novo synthesis inhibitor and pyrimidine de novo synthesis inhibitor: 1
- de novo synthesis inhibitor and synthesis inhibitor: 1, 2, 3
- dmem medium and drug treatment: 1
- drug treatment and synthesis inhibitor: 1, 2, 3, 4, 5
- pyrimidine de novo synthesis inhibitor and synthesis inhibitor: 1
Co phrase search for related documents, hyperlinks ordered by date