Evaluation of Cell-Based and Surrogate SARS-CoV-2 Neutralization Assays

Sholukh, Anton M.; Fiore-Gartland, Andrew; Ford, Emily S.; Miner, Maurine D.; Hou, Yixuan J.; Tse, Longping V.; Kaiser, Hannah; Zhu, Haiying; Lu, Joyce; Madarampalli, Bhanupriya; Park, Arnold; Lempp, Florian A.; St. Germain, Russell; Bossard, Emily L.; Kee, Jia Jin; Diem, Kurt; Stuart, Andrew B.; Rupert, Peter B.; Brock, Chance; Buerger, Matthew; Doll, Margaret K.; Randhawa, April Kaur; Stamatatos, Leonidas; Strong, Roland K.; McLaughlin, Colleen; Huang, Meei-Li; Jerome, Keith R.; Baric, Ralph S.; Montefiori, David; Corey, Lawrence

Determinants of protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection require the development of well-standardized, reproducible antibody assays. This need has led to the emergence of a variety of neutralization assays. Head-to-head evaluation of different SARS-CoV-2 neutralization platforms could facilitate comparisons across studies and laboratories. Five neutralization assays were compared using 40 plasma samples from convalescent individuals with mild to moderate coronavirus disease 2019 (COVID-19): four cell-based systems using either live recombinant SARS-CoV-2 or pseudotyped viral particles created with lentivirus (LV) or vesicular stomatitis virus (VSV) packaging and one surrogate enzyme-linked immunosorbent assay (ELISA)-based test that measures inhibition of the spike protein receptor binding domain (RBD) binding its receptor human angiotensin converting enzyme 2 (hACE2). Vero cells, Vero E6 cells, HEK293T cells expressing hACE2, and TZM-bl cells expressing hACE2 and transmembrane serine protease 2 were tested. All cell-based assays showed 50% neutralizing dilution (ND(50)) geometric mean titers (GMTs) that were highly correlated (Pearson r = 0.81 to 0.89) and ranged within 3.4-fold. The live virus assay and LV pseudovirus assays with HEK293T/hACE2 cells showed very similar mean titers, 141 and 178, respectively. ND(50) titers positively correlated with plasma IgG targeting SARS-CoV-2 spike protein and RBD (r = 0.63 to 0.89), but moderately correlated with nucleoprotein IgG (r = 0.46 to 0.73). ND(80) GMTs mirrored ND(50) data and showed similar correlation between assays and with IgG concentrations. The VSV pseudovirus assay and LV pseudovirus assay with HEK293T/hACE2 cells in low- and high-throughput versions were calibrated against the WHO SARS-CoV-2 IgG standard. High concordance between the outcomes of cell-based assays with live and pseudotyped virions enables valid cross-study comparison using these platforms.