Selected article for: "qpcr reaction and RT qPCR reaction"

Author: Haran Shani-Narkiss; Omri David Gilday; Nadav Yayon; Itamar Daniel Landau
Title: Efficient and Practical Sample Pooling High-Throughput PCR Diagnosis of COVID-19
  • Document date: 2020_4_7
  • ID: 6ji8dkkz_8
    Snippet: (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.06.20052159 doi: medRxiv preprint tube tested requires chemical reagents that are increasingly in short supply as the number of PCR reactions performed globally grows tremendously 11,12 . Laboratories have begun to demonstrate that SARS-CoV-2 can be detected in RT-qPCR performed on pooled samples, despite potential dilution 2 . The input to pool.....
    Document: (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.06.20052159 doi: medRxiv preprint tube tested requires chemical reagents that are increasingly in short supply as the number of PCR reactions performed globally grows tremendously 11,12 . Laboratories have begun to demonstrate that SARS-CoV-2 can be detected in RT-qPCR performed on pooled samples, despite potential dilution 2 . The input to pooling methods is RNA extracted from samples individually, although they may be also used to combine "raw" patient samples, even before lysis and extraction. Their aim is to identify the presence of viral RNA without the need to perform the RT-qPCR reaction on every sample individually.

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