Author: Haran Shani-Narkiss; Omri David Gilday; Nadav Yayon; Itamar Daniel Landau
Title: Efficient and Practical Sample Pooling High-Throughput PCR Diagnosis of COVID-19 Document date: 2020_4_7
ID: 6ji8dkkz_70
Snippet: According to the estimated value of p, use Table 2 to decide on the optimal initial batch size. Process all batches/pools through PCR. All samples from batches that were shown to be negative could be all regarded as negatives. Batches that were shown to be positive should be further divided into equal sizes (for odd numbers just chose arbitrary one batch to be bigger than the other by 1 sample), until all positive samples are isolated. In practic.....
Document: According to the estimated value of p, use Table 2 to decide on the optimal initial batch size. Process all batches/pools through PCR. All samples from batches that were shown to be negative could be all regarded as negatives. Batches that were shown to be positive should be further divided into equal sizes (for odd numbers just chose arbitrary one batch to be bigger than the other by 1 sample), until all positive samples are isolated. In practice, positive batches of size 4 or smaller should not be divided, but simply tested individually.
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