Selected article for: "ccd camera and final concentration"
Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR)
  • Document date: 2019_3_21
    • ID: 9utbwy5r_56
    Snippet: Glass Bottom Microwell Dishes (Part No: P35G-1.5-14C) with 2 mL of DMEM + 10% or 5% 557 FBS, respectively. The next day, the cell media was treated with 10 µL water, 10 mM NH 4 Cl 558 (final concentration), or 60 µM chloroquine (final concentration, Sigma C6628). Four hours after 559 adding inhibitors, DQ Red BSA (Invitrogen Cat #D12051) was added to the media to a final 560 concentration of 5 µg/mL in 2 mL DMEM + 10% or 5% FBS, respectively. .....
    Document: Glass Bottom Microwell Dishes (Part No: P35G-1.5-14C) with 2 mL of DMEM + 10% or 5% 557 FBS, respectively. The next day, the cell media was treated with 10 µL water, 10 mM NH 4 Cl 558 (final concentration), or 60 µM chloroquine (final concentration, Sigma C6628). Four hours after 559 adding inhibitors, DQ Red BSA (Invitrogen Cat #D12051) was added to the media to a final 560 concentration of 5 µg/mL in 2 mL DMEM + 10% or 5% FBS, respectively. At 24 hours post 561 treatment, the cells were imaged on a Nikon TE300 inverted microscope equipped with a 562 mercury arc lamp, Plan-Apochromat 60x, 1.4 NA objective, cooled digital CCD camera (Quantix 563

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    • µg ml and final concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • µg ml and medium add: 1
    • µg ml and NA objective: 1, 2
    • µL water and cell medium: 1
    • µL water and final concentration: 1, 2, 3, 4, 5
    • CCD camera and digital CCD camera: 1, 2
    • CCD camera and final concentration: 1
    • CCD camera and NA objective: 1
    • cell medium and final concentration: 1, 2, 3, 4, 5