Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_101
Snippet: For the accompanying cell viability assay, A549 cells were seeded at 5,000 cells/well in 96-well plates in complete DMEM and incubated for 24h at 37ºC. Cell were then treated with pyrimidine de novo synthesis inhibitors, and/or salvage inhibitors, and/or R1479 in DMEM supplemented with 20 µM uridine. Following 48 h or 72 h treatment, cell viability was monitored via ATP levels, which were measured using the CellTiter-Glo Luciferase Assay System.....
Document: For the accompanying cell viability assay, A549 cells were seeded at 5,000 cells/well in 96-well plates in complete DMEM and incubated for 24h at 37ºC. Cell were then treated with pyrimidine de novo synthesis inhibitors, and/or salvage inhibitors, and/or R1479 in DMEM supplemented with 20 µM uridine. Following 48 h or 72 h treatment, cell viability was monitored via ATP levels, which were measured using the CellTiter-Glo Luciferase Assay System (Promega) according to the specifications of the manufacturer.
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