Selected article for: "linear gradient and reaction neutralize"
Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism
  • Document date: 2020_3_25
    • ID: 1zk64gsg_78
    Snippet: To intermediate S2 (6 mg, 0.023 mmol, 1 equiv.) was added THF (250 µL) and concentrated HCl (25 µL). The reaction mixture was stirred at room temperature for 5 h. An excess amount of NaHCO3 was added to neutralize the reaction, followed by the addition of 1 mL MeOH. The resulting suspension was then filtered through a short Celite pad, rinsed with 2 ´ 2 MeOH, and concentrated. The resulting crude was resuspended with 1 mL H2O and subjected to .....
    Document: To intermediate S2 (6 mg, 0.023 mmol, 1 equiv.) was added THF (250 µL) and concentrated HCl (25 µL). The reaction mixture was stirred at room temperature for 5 h. An excess amount of NaHCO3 was added to neutralize the reaction, followed by the addition of 1 mL MeOH. The resulting suspension was then filtered through a short Celite pad, rinsed with 2 ´ 2 MeOH, and concentrated. The resulting crude was resuspended with 1 mL H2O and subjected to HPLC purification with a linear gradient of 5-20% MeCN (0.1% TFA) in H2O (0.01% TFA) on a prep C18 column (Agilent 10 prep-C18 250 ´ 21.1 mm). Fractions containing desired products were then combined and lyophilized to afford the final product 9 (3.6 mg, 0.014 mmol, 59%) as white powder.

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