Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_45
Snippet: abdominal skin was carefully removed, exposing the peritoneum, which was then injected with 5 436 mL of sterile phosphate-buffered saline (PBS). The abdomen was massaged gently, then the PBS 437 containing the peritoneal macrophages was carefully withdrawn. The macrophages were 438 centrifuged at 100 x g for 4 minutes, red blood cells lysed in lysis buffer (0.15 M ammonium 439 chloride, 1 mM potassium bicarbonate, and 0.1 mM EDTA disodium salt) f.....
Document: abdominal skin was carefully removed, exposing the peritoneum, which was then injected with 5 436 mL of sterile phosphate-buffered saline (PBS). The abdomen was massaged gently, then the PBS 437 containing the peritoneal macrophages was carefully withdrawn. The macrophages were 438 centrifuged at 100 x g for 4 minutes, red blood cells lysed in lysis buffer (0.15 M ammonium 439 chloride, 1 mM potassium bicarbonate, and 0.1 mM EDTA disodium salt) for 2 minutes at room 440 temperature, centrifuged at 100 x g for 4 minutes, washed twice in PBS, resuspended in DMEM 441 + 5% heat-inactivated FBS, and plated in 6 well plates. WT and PKR -/-MEFs (termed PKR WT 442
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