Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_48
Snippet: Kit, Thermo Scientific #23227), were acetone precipitated by incubating with 4x volume ice cold 469 acetone overnight at -20˚C. Precipitated proteins were pelleted at 4˚C at 13,000 x g for 10 470 minutes and the pellets were dried for 30 minutes at room temperature. Pellets were resuspended 471 in 10 µL Pierce TM RIPA lysis buffer (Thermo Scientific #89900), 3.25 µL NuPAGE 4x LDS 472.....
Document: Kit, Thermo Scientific #23227), were acetone precipitated by incubating with 4x volume ice cold 469 acetone overnight at -20˚C. Precipitated proteins were pelleted at 4˚C at 13,000 x g for 10 470 minutes and the pellets were dried for 30 minutes at room temperature. Pellets were resuspended 471 in 10 µL Pierce TM RIPA lysis buffer (Thermo Scientific #89900), 3.25 µL NuPAGE 4x LDS 472
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