Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_12
Snippet: To determine the mechanism of PKR depletion, we first assayed whether reduction in 167 PKR protein during MAV-1 infection was due to reduced PKR mRNA steady-state levels. We 168 mock-infected or infected C57BL/6 MEFs and primary peritoneal macrophages at an MOI of 10 169 and collected cell lysates at 24, 48, and 72 hpi. We synthesized cDNA from RNA purified from 170 these cell lysates and assayed for PKR mRNA by qPCR. In C57BL/6 MEFs, PKR mRNA le.....
Document: To determine the mechanism of PKR depletion, we first assayed whether reduction in 167 PKR protein during MAV-1 infection was due to reduced PKR mRNA steady-state levels. We 168 mock-infected or infected C57BL/6 MEFs and primary peritoneal macrophages at an MOI of 10 169 and collected cell lysates at 24, 48, and 72 hpi. We synthesized cDNA from RNA purified from 170 these cell lysates and assayed for PKR mRNA by qPCR. In C57BL/6 MEFs, PKR mRNA levels 171
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