Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR)

Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler

First, we examined the kinetics of PKR degradation to determine whether an early or late viral 269 protein was likely responsible. We mock infected and infected CMT93 cells with MAV-1 at an 270 MOI of 10 and collected lysates every six hours for 24 hours and analyzed them by immunoblot 271 with antibodies to PKR or MAV-1 early region 1A (E1A) protein, the first viral protein made 272 during infection (68). In infected cells, PKR degradation was first detected at 12 hpi (Fig. 7A) , 273 and quantitation of five independent experiments showed that ~20% of the starting levels of PKR 274 protein remained at 24 hpi ( Fig 7B) . 275 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/584680 doi: bioRxiv preprint