Author: Danielle E. Goodman; Carla D. Pretto; Tomas A. Krepostman; Kelly E. Carnahan; Katherine R. Spindler
Title: Enhanced replication of mouse adenovirus type 1 following virus-induced degradation of protein kinase R (PKR) Document date: 2019_3_21
ID: 9utbwy5r_4
Snippet: To determine whether PKR plays an important role during MAV-1 infection, we tested 128 the susceptibility of both PKR -/-MEF lines to MAV-1 infection. We infected wild type MEFs, 129 N-PKR -/-MEFs, and C-PKR -/-MEFs with MAV-1 at an MOI of 1 PFU/cell and collected cell 130 pellets at 48 and 72 hpi. DNA was purified from the cell pellets and analyzed for MAV-1 131.....
Document: To determine whether PKR plays an important role during MAV-1 infection, we tested 128 the susceptibility of both PKR -/-MEF lines to MAV-1 infection. We infected wild type MEFs, 129 N-PKR -/-MEFs, and C-PKR -/-MEFs with MAV-1 at an MOI of 1 PFU/cell and collected cell 130 pellets at 48 and 72 hpi. DNA was purified from the cell pellets and analyzed for MAV-1 131
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