Selected article for: "linear gradient and stock solution"
Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism
  • Document date: 2020_3_25
    • ID: 1zk64gsg_67
    Snippet: To alcohol 5 (50 mg, 0.13 mmol, 1 equiv.) in 1 mL CH2Cl2 at -78ºC was added a stock solution of DAST (0.15 mmol, 1.2 equiv., 200 µL, prepared by dilute 100 µL of DAST with CH2Cl2 to 1 mL). The reaction mixture was warmed up to room temperature and stirred overnight before quenching with 1 mL saturated NaHCO3. The resulting mixture was then extracted with 3´3 mL EtOAc. The combined organic layers were dried over anhydrous Na2SO4, concentrated,.....
    Document: To alcohol 5 (50 mg, 0.13 mmol, 1 equiv.) in 1 mL CH2Cl2 at -78ºC was added a stock solution of DAST (0.15 mmol, 1.2 equiv., 200 µL, prepared by dilute 100 µL of DAST with CH2Cl2 to 1 mL). The reaction mixture was warmed up to room temperature and stirred overnight before quenching with 1 mL saturated NaHCO3. The resulting mixture was then extracted with 3´3 mL EtOAc. The combined organic layers were dried over anhydrous Na2SO4, concentrated, loaded onto a 4 g SiO2 flash cartridge, and purified with a linear gradient 20-50% EtOAc in hexanes to afford the intermediate S1 (27 mg , 2989, 2937, 1746, 1704, 1667, 1599, 1441, 1374, 1239, 1179, 1060,989 7, 162.2, 149.6, 141.5, 135.4, 131.5, 130.7, 129.4, 123.2, 113.2, 102.7, 95.7, 84.1, 83.2, 80.5 (d, J = 149 Hz), 78.8, 77.4, 77.2, 76.9, 69.2, 27.3, 25.8. 19

    Search related documents:
    Co phrase search for related documents
    • combined organic layer and reaction mixture: 1
    • combined organic layer and room temperature: 1
    • flash cartridge and linear gradient: 1, 2, 3, 4, 5
    • flash cartridge and organic layer: 1, 2
    • flash cartridge and reaction mixture: 1, 2, 3, 4
    • flash cartridge and room temperature: 1, 2
    • flash cartridge and stock solution: 1
    • linear gradient and organic layer: 1, 2
    • linear gradient and reaction mixture: 1, 2, 3, 4, 5
    • linear gradient and room temperature: 1, 2, 3, 4
    • linear gradient and stock solution: 1
    • organic layer and reaction mixture: 1, 2, 3, 4
    • organic layer and reaction mixture room temperature warm: 1
    • organic layer and room temperature: 1, 2, 3, 4
    • organic layer and room temperature warm: 1
    • reaction mixture and result mixture: 1
    • reaction mixture and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • reaction mixture and room temperature warm: 1
    • reaction mixture and stock solution: 1, 2, 3