Author: Powell, Fiona; Rothwell, Lisa; Clarkson, Michael; Kaiser, Pete
Title: Development of reagents to study the turkey's immune response: Cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13 Cord-id: er6irjw3 Document date: 2012_6_15
ID: er6irjw3
Snippet: The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and tra
Document: The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-γ synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays.
Search related documents:
Co phrase search for related documents- liver heart and lung liver: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- liver heart and lung liver detect: 1
- liver heart and lymphoid tissue: 1, 2, 3
- liver heart and macrophage activation: 1
- low expression and lung liver: 1, 2, 3, 4, 5
- low expression and lymphoid tissue: 1, 2
- low expression and macrophage activation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- lung liver and lymphoid tissue: 1, 2, 3, 4, 5
- lung liver and macrophage activation: 1
- lymphoid tissue and macrophage activation: 1
Co phrase search for related documents, hyperlinks ordered by date