Selected article for: "detect high sensitivity and high sensitivity"
Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
  • Document date: 2020_4_14
    • ID: n5kpvsvg_20
    Snippet: In summary, we extended the 3'-and 5'-end of the crRNA and discovered an amplified transcleavage activity of LbCas12a when the 3'-end is extended with DNA or RNA. We applied this modified crRNA/LbCas12a system with the optimal conditions to detect PCA3 in simulated urine with high sensitivity. This ENHANCE technology enabled us to detect the DNA/RNA heteroduplex and methylated DNA with unprecedented sensitivity. We further employed this system to.....
    Document: In summary, we extended the 3'-and 5'-end of the crRNA and discovered an amplified transcleavage activity of LbCas12a when the 3'-end is extended with DNA or RNA. We applied this modified crRNA/LbCas12a system with the optimal conditions to detect PCA3 in simulated urine with high sensitivity. This ENHANCE technology enabled us to detect the DNA/RNA heteroduplex and methylated DNA with unprecedented sensitivity. We further employed this system to test a range of target nucleic acids, including ssDNA, dsDNA, and RNA from HIV, HCV, and SARS-CoV-2 without the need for further optimization. These findings are a crucial step towards enhancing detection of nucleic acids and assisting in the diagnosis of various diseases. author/funder. All rights reserved. No reuse allowed without permission.

    Search related documents:
    Co phrase search for related documents
    • crucial step and high sensitivity: 1, 2, 3, 4
    • crucial step and nucleic acid: 1, 2, 3, 4, 5
    • disease diagnosis and high sensitivity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • disease diagnosis and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • disease diagnosis and nucleic acid detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • disease diagnosis and optimal condition: 1
    • disease diagnosis and optimization need: 1
    • disease diagnosis assist and nucleic acid: 1
    • DNA RNA heteroduplex and high sensitivity: 1
    • DNA RNA heteroduplex and nucleic acid: 1, 2, 3
    • DNA RNA heteroduplex and nucleic acid detection: 1
    • DNA RNA heteroduplex detect and nucleic acid: 1
    • DNA RNA heteroduplex detect and nucleic acid detection: 1
    • ENHANCE technology and nucleic acid: 1
    • high sensitivity and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • high sensitivity and nucleic acid detection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • high sensitivity and optimization need: 1
    • nucleic acid and optimization need: 1