Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection Document date: 2020_4_14
ID: n5kpvsvg_39
Snippet: To find limit of detection (LoD), the fluorophore-quencher reporter assay was carried out with various concentrations of activator. The LoD calculations were based on the following formula 32 : LoD = 3.3 * std of RFU in the absence of activator slope of RFU vs. Activator concentration.....
Document: To find limit of detection (LoD), the fluorophore-quencher reporter assay was carried out with various concentrations of activator. The LoD calculations were based on the following formula 32 : LoD = 3.3 * std of RFU in the absence of activator slope of RFU vs. Activator concentration
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