Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_19
Snippet: Despite its UCK2 inhibitory activity in vitro, 5'-F-CPU did not deplete intracellular nucleotide levels and even appeared to increase levels of some intracellular nucleotides ( Figure 4A and Figure S4 ). This contrast suggests that the activity of CPU and 5-F-CPU is derived primarily from their flux through pyrimidine metabolism pathways and not from their inhibition of UCK2. In all assays, the culture medium was supplemented with 5 µM uridine a.....
Document: Despite its UCK2 inhibitory activity in vitro, 5'-F-CPU did not deplete intracellular nucleotide levels and even appeared to increase levels of some intracellular nucleotides ( Figure 4A and Figure S4 ). This contrast suggests that the activity of CPU and 5-F-CPU is derived primarily from their flux through pyrimidine metabolism pathways and not from their inhibition of UCK2. In all assays, the culture medium was supplemented with 5 µM uridine and 1 µM GSK983. Error bars represent ± S.D. of three biological replicates with statistical comparisons to the untreated control provided for UTP levels (blue) and CTP levels (red) as not significant (n.s), * (p <0.05), ** (p < 0.01), *** (p < 0.001) and **** (p < 0.0001) as calculated by one way ANOVA, Dunnett's test. (B) LC-MS detection of mono-, di-and triphosphates of CPU and 5-F-CPU in cells.
Search related documents:
Co phrase search for related documents- CPU cpu and culture medium: 1, 2
- CPU cpu and inhibitory activity: 1, 2
- CPU cpu and intracellular nucleotide: 1
- culture medium and inhibitory activity: 1, 2, 3, 4, 5
- culture medium and intracellular nucleotide: 1
- inhibitory activity and LC MS detection: 1, 2
Co phrase search for related documents, hyperlinks ordered by date