A nematode-specific gene underlies bleomycin-response variation in Caenorhabditis elegans

Shannon C. Brady; Stefan Zdraljevic; Karol W. Bisaga; Robyn E. Tanny; Daniel E. Cook; Daehan Lee; Ye Wang; Erik C. Andersen

Given that the QTL explained 43.58% of phenotypic variation among the RIAILs, the causal 557 variant should have a clear impact on bleomycin response. Additionally, the NILs with the N2 558 allele at the QTL introgressed into the CB4856 background displayed a significant increase in 559 bleomycin sensitivity compared to the parental CB4856 strain (Figure S6, File S7) . Taken 560 together, the phenotypes of the reciprocal allele-replacement strains showed that the amino-acid 561 change in JMJD-5 likely does not underlie bleomycin-response variation between the N2 and 562 . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/565218 doi: bioRxiv preprint 26 of 48 CB4856 strains, although deletion of this gene did cause resistance to bleomycin regardless of 563 the genetic background. We performed a reciprocal hemizygosity assay to test if natural variation 564 in jmjd-5 function affected bleomycin responses ( Figure S10, Figure S11 , Figure S12 ). The 565 results of this assay supported the previously identified increase in bleomycin resistance of 566 homozygous jmjd-5 deletions in both parental backgrounds ( Figure S11, File S7 , p < 0.05, 567