Author: Gloria P. Larson; Vy Tran; Shuiqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle
Title: EPS8 facilitates uncoating of influenza A virus Document date: 2019_3_28
ID: muq5rkaa_46
Snippet: Interactions between EPS8 and incoming RNPs was investigated in EPS8.1 A549 cells stably complemented with EPS8-V5. Cells were inoculated with WSN at an MOI of 25 diluted in cold VGM. Infections were synchronized by inoculating cells at 4˚C for 1 hour. Warm VGM was added to the cells and infection progressed for 2.5 hours at 37°C. Cells were washed with cold PBS and lysed in co-IP buffer (50 mM Tris pH 7.4, 150 mM NaCl, 0.5% NP-40) supplemented.....
Document: Interactions between EPS8 and incoming RNPs was investigated in EPS8.1 A549 cells stably complemented with EPS8-V5. Cells were inoculated with WSN at an MOI of 25 diluted in cold VGM. Infections were synchronized by inoculating cells at 4˚C for 1 hour. Warm VGM was added to the cells and infection progressed for 2.5 hours at 37°C. Cells were washed with cold PBS and lysed in co-IP buffer (50 mM Tris pH 7.4, 150 mM NaCl, 0.5% NP-40) supplemented with protease inhibitors. Lysates were clarified and subjected to immunoprecipitation with 1 μg anti-V5 antibody or control rabbit IgG. Immune complexes were captured with protein A agarose resin, washed extensively with co-IP buffer, eluted, and analyzed by anti-RNP immunoblot to probe for NP.
Search related documents:
Co phrase search for related documents- cell inoculate and infection cell: 1
- control antibody and infection cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
Co phrase search for related documents, hyperlinks ordered by date