Selected article for: "Ct cycle threshold and virus detect"

Author: Pilarowski, Genay; Lebel, Paul; Sunshine, Sara; Liu, Jamin; Crawford, Emily; Marquez, Carina; Rubio, Luis; Chamie, Gabriel; Martinez, Jackie; Peng, James; Black, Douglas; Wu, Wesley; Pak, John; Laurie, Matthew T.; Jones, Diane; Miller, Steve; Jacobo, Jon; Rojas, Susana; Rojas, Susy; Nakamura, Robert; Tulier-Laiwa, Valerie; Petersen, Maya; Havlir, Diane V.; DeRisi, Joseph
Title: Performance characteristics of a rapid SARS-CoV-2 antigen detection assay at a public plaza testing site in San Francisco
  • Cord-id: fatm351r
  • Document date: 2020_11_4
  • ID: fatm351r
    Snippet: We evaluated the performance of the Abbott BinaxNOW™ Covid-19 rapid antigen test to detect virus among persons, regardless of symptoms, at a public plaza site of ongoing community transmission. Titration with cultured clinical SARS-CoV-2 yielded a human observable threshold between 1.6×10(4)-4.3×10(4) viral RNA copies (cycle threshold (Ct) of 30.3–28.8 in this assay). Among 878 subjects tested, 3% (26/878) were positive by RT-PCR, of which 15/26 had a Ct<30, indicating high viral load. 40%
    Document: We evaluated the performance of the Abbott BinaxNOW™ Covid-19 rapid antigen test to detect virus among persons, regardless of symptoms, at a public plaza site of ongoing community transmission. Titration with cultured clinical SARS-CoV-2 yielded a human observable threshold between 1.6×10(4)-4.3×10(4) viral RNA copies (cycle threshold (Ct) of 30.3–28.8 in this assay). Among 878 subjects tested, 3% (26/878) were positive by RT-PCR, of which 15/26 had a Ct<30, indicating high viral load. 40% (6/15) of Ct<30 were asymptomatic. Using this Ct<30 threshold for Binax-CoV2 evaluation, the sensitivity of the Binax-CoV2 was 93.3% (14/15), 95% CI: 68.1–99.8%, and the specificity was 99.9% (862/863), 95% CI: 99.4–99.9%.

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