Author: Gloria P. Larson; Vy Tran; Shuiqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle
Title: EPS8 facilitates uncoating of influenza A virus Document date: 2019_3_28
ID: muq5rkaa_30
Snippet: Authenticated stocks 293T (#CRL-3216), A549 (#CCL-185), and MDCK (#CCL-34) were purchased from the American Type Culture Collection. Parental and edited 293 cells were obtained from Synthego. MDCK-HA cells were a gift from P. Palese (Marsh et al., 2007) . All cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Corning cellgro) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Atlanta Biosciences) and grown at 37°C wi.....
Document: Authenticated stocks 293T (#CRL-3216), A549 (#CCL-185), and MDCK (#CCL-34) were purchased from the American Type Culture Collection. Parental and edited 293 cells were obtained from Synthego. MDCK-HA cells were a gift from P. Palese (Marsh et al., 2007) . All cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Corning cellgro) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Atlanta Biosciences) and grown at 37°C with 5% CO2. Cells were regularly tested and verified free of mycoplasma contamination using MycoAlert (Lonza, LT07-218). The NCI-60 cell lines are a panel of 59 human breast, central nervous system (CNS), colon, lung, melanoma, ovarian renal cancer, and prostate cancer cell lines (Weinstein, 2006) . The NCI-60 panel was obtained from the US National Cancer Institute's Developmental Therapeutics Program (NCI DTP), Fort Detrick, Frederick, MD, USA. All NCI-60 panel cell lines were grown in RPMI-1640 (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS (Sigma-Aldrich) at 37°C in a humidified 5% CO2 atmosphere. Transfection of 293T and 293 cells was conducted using TransIT-2020 (Mirus, MIR 5400).
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