Establishing Prokaryotic Expression System of Angiotensin-Converting Enzyme 2 (ACE2) gene in pigs

Hang Xiao; Xin-Tian Nie; Xiao-Xia Ji; Shu-ping Yan; Bin Zhu; Yuan-Shu Zhang

From the tree of genetic evolution (Figure 52-B) , the fragment is in the same 237 evolutionary group with the ACE2 amino acid sequence of goats and cattle, and the 238 zebrafish is in a completely different 2 branch, and the genetic evolution is consistent . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.12.988634 doi: bioRxiv preprint 239 with the evolution of amino acid. proteins, such as people and sheep, were the same. Among them, part 1~739aa is the 245 extracellular part, and there is a transmembrane helix between 740~762 aa, and the 246 763~805 AA is the intracellular part (Fig. 3-A) . The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.12.988634 doi: bioRxiv preprint pET-32a-ACE2 recombinant plasmid was successfully constructed. Results as shown in Figure 7 , the serum dilution ratio of the positive serum OD 450 was 288 about 1, and the P/N value (P: the mean OD 450 value of the positive hole, the mean 289 value of the negative pore, OD 450 ) was the best serum dilution degree, that was 3200.