Author: Mori, Julie; Clewley, Jonathan P.
Title: Polymerase chain reaction and sequencing for typing rhinovirus RNA Cord-id: dbybnpsh Document date: 2005_12_7
ID: dbybnpsh
Snippet: Primers were designed and tested for their ability to distinguish rhinoviruses from enteroviâ€ruses. A primer set derived from the 5′â€UTR/VP coding region junction was able to amplify all the rhinovirus serotypes tested. Enteroviruses were either not amplified by these primer pairs or produced a band of larger size that could easily be discriminated from the rhinovirusâ€specific product. In contrast, primers embedded in the 5′â€UTR region alone were able to amplify both rhinovirus and e
Document: Primers were designed and tested for their ability to distinguish rhinoviruses from enteroviâ€ruses. A primer set derived from the 5′â€UTR/VP coding region junction was able to amplify all the rhinovirus serotypes tested. Enteroviruses were either not amplified by these primer pairs or produced a band of larger size that could easily be discriminated from the rhinovirusâ€specific product. In contrast, primers embedded in the 5′â€UTR region alone were able to amplify both rhinovirus and enterovirus RNA. It is shown that rhinoâ€viruses could be specifically typed by sequencing the amplicon derived from this 5′â€UTR set. The sequences of the 5′â€UTR region often previously unsequenced rhinoviruses were derived. The sequences obtained cluster into two groups: 18,41, 15, 30, 63, 31,56, and 44; and 17, 69, and 70. Ampliconsfrom serotypes 17, 69, and 70 also group by sequence with the equivalent region of HRV14 from the genetic database, while the others group with 2 and 89. © 1994 Wileyâ€Liss, inc.
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