Author: Gloria P. Larson; Vy Tran; Shuiqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle
Title: EPS8 facilitates uncoating of influenza A virus Document date: 2019_3_28
ID: muq5rkaa_32
Snippet: Antibodies used (Tran et al., 2015) , WSN with the polymerase from A/green-winged teal/ OH/175/1983 (H2N1) encoding PB2 S590/R591/K627 (S009-SRK PASTN) (Tran et al., 2015) , B/Brisbane/60/2008 (B/Brisbane) PASTN, and FVG-R (Hao et al., 2008) were rescued using the influenza virus reverse genetics system and prepared as previously described. WSN PASN was further purified by centrifugation through a 20% sucrose cushion to remove contaminating lucif.....
Document: Antibodies used (Tran et al., 2015) , WSN with the polymerase from A/green-winged teal/ OH/175/1983 (H2N1) encoding PB2 S590/R591/K627 (S009-SRK PASTN) (Tran et al., 2015) , B/Brisbane/60/2008 (B/Brisbane) PASTN, and FVG-R (Hao et al., 2008) were rescued using the influenza virus reverse genetics system and prepared as previously described. WSN PASN was further purified by centrifugation through a 20% sucrose cushion to remove contaminating luciferase present in the media (Tran et al., 2015) . WSN-GFP was amplified and titered on HA-MDCK cells (Marsh et al., 2007) .
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