Building Your Own Neuroscience Equipment: A Precision Micromanipulator and an Epi-fluorescence Microscope for Calcium Imaging.

Ryan, James; Johnson, Bruce R; Deitcher, David

A faculty member's ability to develop meaningful research-oriented laboratories in neurobiology is often hampered by the rapid pace of new technologies and the increasing cost of equipment. To help undergraduate neuroscience faculty meet these challenges, we introduce two important neuroscience research tools we designed and built. The first is a precision micromanipulator for neurophysiology applications costing less than $40 USD. We compare data generated using the DIY manipulator with commercial micromanipulators costing over $1000. The second tool is our newly designed 3D printed epi-fluorescence microscope. Commercial fluorescence imaging devices often cost over $20,000, but our 3D printed version is constructed for less than $1200. This epi-fluorescence microscope uses interchangeable LED light sources and filter sets to image static fluorescence in prepared slides and calcium imaging of neuronal activity in living Drosophila brains. This later technique uses transgenic flies with a genetically encoded calcium indicator, GCaMP, linked to green fluorescent protein (GFP). During an action potential, calcium ions (Ca2+) enter neurons and are observed as an increase in fluorescence intensity from a series of video images. These neuronal firing patterns can be assessed qualitatively and quantitatively to understand neural circuits leading to specific behaviors. We plan to develop curricula around the use of the epi-fluorescence microscope for calcium imaging in the next year, and to provide detailed parts sources and construction guides for the student and faculty DIY experience.